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Novus Biologicals
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Jackson Immuno
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US Biological Life Sciences
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Synaptic Systems
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Amersham Life Sciences Inc
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Image Search Results
Journal: Frontiers in molecular neuroscience
Article Title: Specific Role for GSK3α in Limiting Long-Term Potentiation in CA1 Pyramidal Neurons of Adult Mouse Hippocampus.
doi: 10.3389/fnmol.2022.852171
Figure Lengend Snippet: FIGURE 1 | Spatial validation of GSK3α or GSK3β cKO by IHF. (A) Fluorescence images of brain sections through the hippocampus collected with a 10 × objective lens following staining with anti-GSK3α (revealed with Alexa 594) and anti-GSK3β (revealed with Cy5), as well as DNA using DAPI; the three columns on each row is from a single section imaged sequentially in the corresponding fluorescence wavelength channels. IHF from each of a GSK3α cKO, GSK3β cKO, and a Cre- subject are shown in rows. Note the prominent reduction in corresponding GSK3 staining within the CA1 region of each paralog cKO. (B) The white outlined zones in A were subsequently imaged with a 60 × lens and confocal optical resolution. The same fluorescence channels are presented, plus a final merged overlay in the final column. (C) Primary antibody was omitted, and images from the same Cre- subject were collected at the exact same settings as those used in (A). (A–C) Images shown were selected from a sampling of n = 2–3 subjects per genotype, and are representative from a total of 36 images collected with a 10 × objective lens, and 82 images collected with a 60 × objective lens.
Article Snippet: Slides were washed with PBS 3 × 5 min, incubated with secondary antibodies, donkey anti-rabbit conjugated with Alexa Fluor 594 (Jackson ImmunoResearch Inc, West Grove, PA, United States, Cat# 711- 585-152) and
Techniques: Biomarker Discovery, Fluorescence, Staining, Sampling
Journal: Frontiers in Cellular Neuroscience
Article Title: Immunosignals of Oligodendrocyte Markers and Myelin-Associated Proteins Are Critically Affected after Experimental Stroke in Wild-Type and Alzheimer Modeling Mice of Different Ages
doi: 10.3389/fncel.2018.00023
Figure Lengend Snippet: Double and triple fluorescence labeling.
Article Snippet: Rabbit-anti-claudin 11/OSP (1:400; 241 003; Synaptic Systems) , Cy3-donkey-anti-rabbit IgG (711-165-152) , Guinea pig-anti-MBP (1:200; 295 004;
Techniques: Fluorescence, Labeling, Marker, Plasmid Preparation